Abstract - Potential applications of modern reproductive technologies including monitoring follicular dynamics, es- trus synchronization and superovulation would be a new dimension of research at the farm level for improving buffalo’s fertility and production. Therefore, the study was aimed to understand and monitor the ovarian dy- namics after induction of superovulation in buffalo cows. Ovarian activities after superovulation treatment were monitored by trans-rectal ultrasonography. Follicle stimulating hormone (FSH) was used for superovulation in six mature Murrah buffalo cows and the embryos were collected by non-surgically technique on day 6 of the estrous cycle. The result showed a significant (P < 0.05) effect of superovulation treatment on follicular size, number and maturation. The result also revealed that the number and size of follicles of right and left ovaries, as well as their growth rate, have no significant (P > 0.05) difference during the superovulation treatment of animals. The development of dominant follicle and formation of corpus luteum after treating with the FSH had shown positive results in the present study but the number of recovery of embryos was very poor. Observed the ovulation rate was 58.62% and the embryo recovery rate was very poor (? 3%). This study suggests that more efforts should be made to better comprehend the mechanism of embryo production, maturation and collection during the MOET program in buffalo cows.