Abstract - This study was designed to evaluate the effects of Xylazine-Ketamine and Ketamine alone on clinical pa- rameters (rectal temperature, heart rate, respiratory rate and SpO2), haematological parameters (TEC, TLC, Hb and PCV) and biochemical parameters (AST, ALT, BUN, TP and Creatinine) during general anesthesia in sheep. Six healthy sheep were used for this experiment and divided into two groups named, Group A (Xylazine- Ketamine) and Group B (Only Ketamine). All the parameters measured and recorded before anesthesia, at 5 min, 10 min, 15 min, 20 min, 25 min, 30 min, hour post-induction of anaesthesia and after 24 hours of recovery. Temperature decreased significantly (P < 0.05) from control value at 10 min post-induction in both group A and B. This decrement continued up to 25 min post-induction and then started to increase. Heart rate increased (P < 0.05) significantly from control value at 10 min post-induction in sheep of Group A. This increment con- tinued up to 25 min post-induction and then started to decrease. But in Group B, after 10 min of induction, heart rate fluctuated remarkably. Respiratory rate was decreased after 5 min post-induction and it was in the same trend up to 25 min. We also found fluctuation on the respiratory rate in Group B. We observed a decreasing pattern on SpO2 in both groups. In haematological parameters, we found a significant decreasing trend in all parameters up to 25 min post-induction in both groups. We found non-significant decrement of ALT and AST up to 25 min post-induction in both groups. The BUN and TP value were significantly (P < 0.05) decreased from control value up to 25 min post-induction in Group A, but in Group B value changed with a fluctuated trend. We got an increment on creatinine value in both groups, but in the case of Group B, the increment was high. Results suggest that combination of Xylazine-Ketamine can be used for general anesthesia in sheep rather than Ketamine alone although there are some systemic effects on the body.